MECP2 antibody (AA 119-453)

Catalog No. ABIN8093317

This Rabbit Polyclonal antibody specifically detects MECP2 in WB, IHC, ELISA, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MECP2 antibody (AA 119-453) (ABIN8093317)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MECP2 Antibody

Binding Specificity: AA 119-453

Purpose: MECP2 Antibody / Methyl-CpG-binding protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MECP2 recombinant protein (Position: K119-R453) was used as the immunogen for Methyl-CpG-binding protein 2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Methyl-CpG-binding protein 2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Methyl-CpG-binding protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2

Background: Methyl-CpG-binding protein 2 antibody targets Methyl-CpG-binding protein 2, encoded by the MECP2 gene. Methyl-CpG-binding protein 2 is a nuclear chromatin-associated protein that binds methylated CpG dinucleotides and functions as a regulator of gene expression. MECP2 plays a central role in interpreting DNA methylation marks and linking epigenetic modifications to transcriptional control.

Functionally, Methyl-CpG-binding protein 2 acts primarily as a transcriptional regulator by recruiting corepressor complexes and chromatin-modifying enzymes to methylated DNA regions. Through these interactions, MECP2 influences chromatin structure and gene accessibility, contributing to long-term regulation of gene expression rather than rapid transcriptional switching. A MECP2 antibody supports studies focused on epigenetic regulation, chromatin biology, and transcriptional repression mechanisms.

MECP2 expression is particularly prominent in neuronal cells, where it is required for normal brain development and synaptic function. Subcellular localization is predominantly nuclear, consistent with its role in chromatin binding, although distribution within the nucleus can vary depending on chromatin state and developmental context. MECP2 abundance and activity are tightly regulated, reflecting its importance in maintaining transcriptional homeostasis.

From a disease-relevance perspective, mutations or altered regulation of MECP2 are strongly associated with neurodevelopmental disorders, most notably Rett syndrome. Dysregulation of MECP2-dependent gene expression programs can disrupt neuronal maturation and function, highlighting the protein's critical role in nervous system development and maintenance. MECP2 has also been investigated in broader contexts of epigenetic dysfunction and transcriptional misregulation.

At the molecular level, Methyl-CpG-binding protein 2 contains a methyl-CpG-binding domain responsible for recognizing methylated DNA, along with transcriptional repression domains that mediate protein-protein interactions. Isoform variation and post-translational regulation can influence MECP2 behavior in experimental systems without altering its core DNA-binding function. MECP2 antibody reagents support research applications focused on epigenetic control and nuclear gene regulation, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane