Recombinant Perforin 1 antibody (AA 400-555)

Catalog No. ABIN8093399

The Rabbit Monoclonal anti-Perforin 1 antibody is suitable to detect Perforin 1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Perforin 1 antibody (AA 400-555) (ABIN8093399)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: PRF1-9681R

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 400-555

Purpose: Perforin Antibody / PRF1

Immunogen: A recombinant fragment (around amino acids 400-555) of human Perforin-1 protein (exact sequence is proprietary) was used as the immunogen for the Cytotoxic Lymphocyte Perforin/PRF1 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Cytotoxic Lymphocyte Perforin/PRF1 antibody should be determined by the researcher.

2. This Cytotoxic Lymphocyte Perforin/PRF1 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Cytotoxic Lymphocyte Perforin/PRF1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Cytotoxic Lymphocyte Perforin Antibody recognizes Perforin, a calcium-dependent pore-forming protein encoded by the PRF1 gene. Perforin is a critical effector molecule of cytotoxic T lymphocytes and natural killer cells, where it is synthesized, stored in secretory granules, and released upon immune synapse formation. Following antigen recognition, Perforin is exocytosed and inserts into the membrane of target cells, forming transmembrane pores that allow granzymes to enter and initiate apoptosis. This tightly regulated mechanism is central to antiviral defense, tumor immunosurveillance, and elimination of intracellular pathogens.

Perforin belongs to the membrane attack complex/perforin (MACPF) protein family and contains a conserved MACPF domain responsible for pore formation. The protein also includes calcium-binding regions that regulate its conformational change and membrane insertion. Under resting conditions, Perforin remains sequestered within cytotoxic granules to prevent unintended tissue damage. Upon activation of cytotoxic lymphocytes, controlled secretion ensures targeted killing of infected or transformed cells while minimizing collateral injury to surrounding tissues.

The PRF1 gene is located on chromosome 10 and is predominantly expressed in activated CD8-positive T cells and natural killer cells. In lymphoid organs such as spleen, tonsil, and lymph node, Perforin-positive cells are typically scattered within germinal centers and interfollicular regions, reflecting the distribution of activated cytotoxic lymphocytes. Cytoplasmic granular staining is the expected localization pattern in immunohistochemistry due to storage within lytic granules.

Genetic mutations in PRF1 are associated with familial hemophagocytic lymphohistiocytosis and other immune dysregulation syndromes, highlighting the essential role of Perforin in maintaining immune homeostasis. Altered expression levels have also been studied in cancer immunology, transplant rejection, and autoimmune disease research. Quantifying Perforin expression can provide insight into cytotoxic immune activity within tumor microenvironments or inflammatory lesions.

This Cytotoxic Lymphocyte Perforin Antibody (clone PRF1/9681R) is a recombinant monoclonal antibody suitable for detecting PRF1 expression in research applications. A Cytotoxic Lymphocyte Perforin Antibody supports studies of cell-mediated cytotoxicity, immune activation status, and functional characterization of cytotoxic lymphocyte populations in normal and diseased tissues.

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis