Recombinant Melanoma gp100 antibody (AA 561-661)

Catalog No. ABIN8093420

The Rabbit Monoclonal anti-Melanoma gp100 antibody is suitable to detect Melanoma gp100 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Melanoma gp100 antibody (AA 561-661) (ABIN8093420)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PMEL-7554R

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: AA 561-661

Purpose: PMEL17 / Melanoma gp100 Antibody

Purification: Protein A affinity

Immunogen: A recombinant fragment (around amino acids 561-661) of human SILV protein (exact sequence is proprietary) was used as the immunogen for the Melanocyte Lineage/PMEL antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Melanocyte Lineage/PMEL antibody should be determined by the researcher.

2. This Melanocyte Lineage/PMEL antibody is recombinantly produced by expression in human HEK293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Melanocyte Lineage/PMEL antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL17 / Melanoma gp100

Background: Premelanosome protein (PMEL) is a melanocyte lineage-associated glycoprotein encoded by the PMEL gene that functions as a key structural component of developing melanosomes where melanin deposition occurs. The protein is widely known in the literature as gp100 or Pmel17 and plays an essential role in the formation of fibrillar matrices that organize pigment polymerization within melanosomes. Melanocyte Lineage Antibody / PMEL (clone PMEL/7554R) recognizes this melanocyte-associated protein and supports studies examining gp100 expression in melanocytes and melanoma cells. The protein is frequently referred to in the literature as gp100, Pmel17, or premelanosome protein and is widely used as a melanocytic lineage marker in melanoma and pigment cell biology research.

The gp100 protein participates in early melanosome development where it undergoes proteolytic processing and structural rearrangement to form amyloid-like fibrils that generate the internal matrix of premelanosomes. These fibrils provide the scaffold required for deposition of eumelanin and pheomelanin pigments during melanogenesis. Proper assembly of this fibrillar structure is essential for melanosome architecture and efficient pigment production. Within melanocytes and melanoma cells, gp100 localizes primarily to premelanosomes and early stage melanosomes where pigment granules are organized and matured.

Protein microarray specificity analysis of clone PMEL/7554R screened antibody binding against thousands of human proteins and demonstrated selective recognition of PMEL relative to other proteins present on the array. Large-scale protein microarray screening provides a powerful experimental approach for evaluating antibody specificity by measuring binding across a broad proteomic background. The protein microarray specificity data associated with Melanocyte Lineage Antibody clone PMEL/7554R therefore provides additional evidence supporting the selectivity of this antibody for the intended PMEL target.

In large tissue profiling studies including human tissue microarray (TMA) panels containing multiple normal and cancer tissues, gp100 expression is largely restricted to melanocytes and melanocytic tumors. Tissue microarray analysis consistently shows strong staining of melanoma cells while most non-melanocytic tissues remain negative, reinforcing the value of PMEL as a melanocyte lineage marker in melanoma research and diagnostic pathology investigations.

Melanocyte Lineage Antibody / PMEL clone PMEL/7554R detects the gp100 protein present in melanocytes and melanoma cells and supports investigation of melanosome-associated structural proteins. The availability of protein microarray specificity data combined with well-established melanocyte lineage expression patterns makes this antibody a useful reagent for studies examining melanocyte biology, melanosome formation, and melanoma-associated molecular pathways.

UniProt: P40967