NLRP1 antibody (Middle Region)

Catalog No. ABIN8106572

The Rabbit Polyclonal anti-NLRP1 antibody is suitable to detect NLRP1 in samples from Human. It has been validated for WB.

Quick Overview for NLRP1 antibody (Middle Region) (ABIN8106572)

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NLRP1 Antibody

Binding Specificity: Middle Region

Purpose: NLRP1 Antibody - middle region

Sequence: LCSLAAEGIW QKKTLFSPDD LRKHGLDGAI ISTFLKMGIL QEHPIPLSYS

Predicted Reactivity: Cow: 85%, Dog: 100%, Horse: 79%, Human: 100%

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NLRP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54479-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NLRP1

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Alternative Name: NLRP1

Background:

Background Information: This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Gene Name: NLR family pyrin domain containing 1

Alternative Symbols: NAC, JRRP, MSPC, AIADK, CARD7, CIDED, NALP1, SLEV1, DEFCAP, PP1044, VAMAS1, CLR17.1, DEFCAP-L/S

Protein Name: NACHT, LRR and PYD domains-containing protein 1

Molecular Weight: 157 kDa

Gene ID: 22861

NCBI Accession: NP_001028225

UniProt: Q9C000

Pathways: Positive Regulation of Endopeptidase Activity, Inflammasome