FBXW7 antibody

Catalog No. ABIN760916

The Rabbit Polyclonal anti-FBXW7 antibody is suitable to detect FBXW7 in samples from Human. It has been validated for FACS, IF, IHC (p) and IHC (fro).

Quick Overview for FBXW7 antibody (ABIN760916)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FBXW7 Antibody

Purpose: CDC4 Polyclonal Antibody

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FBW7

Isotype: IgG

Application Details

Application Notes: IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1 μg/Test)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBW7

Background:

Synonyms: AGO, Archipelago homolog, Archipelago, Drosophila, homolog of antibody CDC4, DKFZp686F23254, F box and WD 40 domain protein 7 archipelago homolog, Drosophila, F box and WD 40 domain protein 7, F box and WD repeat domain containing 7, F box protein FBW7, F box protein FBX30, F box protein SEL10, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FBXW7_HUMAN, FLJ16457, hAgo, hCdc4, Homolog of C elegans sel 10, Homolog of C.elegans sel10, SEL-10, SEL10.

Background: Fbw7 is a member of the F box protein family which are characterized by an approximately 40 amino acid motif, the F box. The F box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F box), which function in phosphorylation-dependent ubiquitination. The F box proteins are divided into 3 classes: Fbws containing WD40 domains, Fbls containing leucine rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. Fbw7 belongs to the Fbws class, in addition to an F box, this protein contains 7 tandem WD40 repeats. It binds directly to cyclin E and probably targets cyclin E for ubiquitin mediated degradation. Mutations of this gene are detected in ovarian and breast cancer cell lines. Alternative splicing of this gene generates 2 transcript variants diverging at the 5' termini.

Molecular Weight: 69/110kDa

Gene ID: 55294

Pathways: Notch Signaling, EGFR Signaling Pathway