alpha Tubulin antibody (Lys112)

Catalog No. ABIN8119674

The Rabbit Polyclonal anti-alpha Tubulin antibody is suitable to detect alpha Tubulin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for alpha Tubulin antibody (Lys112) (ABIN8119674)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Tubulin Antibody

Binding Specificity: Lys112

Purpose: Tubulin α Antibody

Specificity: Tubulin α Polyclonal Antibody detects endogenous levels of Tubulin α protein only when acetylation at K112.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Tubulin alpha around the non-acetylation site of K112.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Tubulin alpha

Background:

Synonyms: TUBA1A, TUBA3, Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, TUBA1B, Tubulin alpha-1B chain, Alpha-tubulin ubiquitousTubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1C, TUBA6, Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain

Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.

Gene Name: TUBA1A/TUBA1B/TUBA1C

Protein Name: Tubulin alpha-1A chain/Tubulin alpha-1B chain/Tubulin alpha-1C chain

Gene ID: 7846, 10376

UniProt: Q71U36

Pathways: Microtubule Dynamics