AMACR antibody (AA 300-382)

Catalog No. ABIN8119692

The Mouse Monoclonal anti-AMACR antibody is suitable to detect AMACR in samples from Human. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for AMACR antibody (AA 300-382) (ABIN8119692)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: AB1490

Product Details anti-AMACR Antibody

Binding Specificity: AA 300-382

Purpose: AMACR mouse mAb

Specificity: The antibody can specifically recognize human AMACR protein. In western blotting of Hela and LnCap cell lysates, the antibody can label a 42 kDa band corresponding to AMACR.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 300 - 382 of the human AMACR protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:100-1:500 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR

Background:

Synonyms: 2 arylpropionyl CoA epimerase, 2 methylacyl CoA racemase, 2-methylacyl-CoA racemase, Alpha methylacyl CoA racemase, Alpha methylacyl Coenzyme A racemase, Alpha methylacyl-CoA racemase deficiency, included, Alpha-methylacyl-CoA racemase, Amacr, AMACR deficiency, included, AMACR_HUMAN, CBAS4, Da1-8, EC 5. 1. 99. 4, Macr1, Methylacyl CoA racemase alpha, RACE, RM

Background: AMACR is an α Formyl COA racemase localized in peroxisomes and mitochondria . It is an enzyme involved in biosynthesis of bile acid and β-oxidation of branched-chain fatty acids. It is expressed in the epithelium and salivary gland of proximal convoluted tubules of pancreas, liver and kidney, and moderately in paraurethral glands. The expression in prostate cancer is higher than that in benign prostate tissue. In the study of prostate cancer, it is often used in combination with PSA, p63, 34 β E12 and other antibodies.

Gene Name: AMACR

Protein Name: AMACR

Gene ID: 23600

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process