Caspase 1 antibody (AA 350-404)

Catalog No. ABIN8120223

The Mouse Monoclonal anti-Caspase 1 antibody is suitable to detect Caspase 1 in samples from Human, Mouse, Rat, Pig and Horse. It has been validated for WB, ELISA and IF.

Quick Overview for Caspase 1 antibody (AA 350-404) (ABIN8120223)

Target: Caspase 1 (CASP1)

Reactivity: Human, Mouse, Rat, Pig, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB2371

Product Details anti-Caspase 1 Antibody

Binding Specificity: AA 350-404

Purpose: Caspase-1 mouse mAb

Specificity: This antibody detects endogenous levels of Caspase-1 protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 350 - 404 of the human Caspase - 1 protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background:

Synonyms: Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45

Background: caspase 1 (CASP1) Homo sapiens This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012],

Gene Name: CASP1

Protein Name: Caspase-1

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome