CDC25C antibody

Catalog No. ABIN8120442

The Rabbit Monoclonal anti-CDC25C antibody is suitable to detect CDC25C in samples from Human and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for CDC25C antibody (ABIN8120442)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2127

Product Details anti-CDC25C Antibody

Purpose: Cdc25C Rabbit mAb

Specificity: This antibody detects endogenous levels of Cdc25C protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: Cdc25C

Background:

Synonyms: CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C

Background: cell division cycle 25C (CDC25C) Homo sapiens This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015],

Gene Name: CDC25C

Protein Name: Cdc25C

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase