Cyclin E1 antibody (pThr62)

Catalog No. ABIN8120793

The Rabbit Polyclonal anti-Cyclin E1 antibody is suitable to detect Cyclin E1 in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for Cyclin E1 antibody (pThr62) (ABIN8120793)

Target: Cyclin E1 (CCNE1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin E1 Antibody

Binding Specificity: pThr62

Purpose: Cyclin E1 (Phospho Thr62) Antibody

Specificity: This antibody detects endogenous levels of Human Cyclin E1 (phospho-Thr62)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized phosho peptide around human Cyclin E1 (Thr62)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background:

Synonyms: G1/S-specific cyclin-E1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

Gene Name: CCNE1 CCNE

Protein Name: Cyclin E1 (Thr62)

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases