p300 antibody (acLys1542)

Catalog No. ABIN8121099

The Rabbit Polyclonal anti-p300 antibody is suitable to detect p300 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for p300 antibody (acLys1542) (ABIN8121099)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-p300 Antibody

Binding Specificity: acLys1542

Purpose: p300 (Acetyl Lys1542) Antibody

Specificity: Acetyl-p300 (K1542) Polyclonal Antibody detects endogenous levels of p300 around the acetylation site of K1542 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized acetyl-peptide derived from human p300 around the acetylation site of K1542.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300

Background:

Synonyms: EP300, P300, Histone acetyltransferase p300, p300 HAT, E1A-associated protein p300

Background: E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],

Gene Name: EP300

Protein Name: Histone acetyltransferase p300

Gene ID: 2033

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size