E2F2 antibody (acLys122)

Catalog No. ABIN8121110

This Rabbit Polyclonal antibody specifically detects E2F2 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for E2F2 antibody (acLys122) (ABIN8121110)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F2 Antibody

Binding Specificity: acLys122

Purpose: E2F-2 (Acetyl Lys122) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse E2F-2 (Acetyl Lys122)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human E2F-2 (Acetyl Lys122)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F-2

Background:

Synonyms: Transcription factor E2F2 (E2F-2)

Background: function:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP.,tissue specificity:Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.,

Gene Name: E2F2

Protein Name: E2F-2 (Acetyl Lys122)

Gene ID: 1870

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication