ELANE antibody

Catalog No. ABIN8121147

The Rabbit Polyclonal anti-ELANE antibody is suitable to detect ELANE in samples from Human, Mouse and Cow. It has been validated for WB and ELISA.

Quick Overview for ELANE antibody (ABIN8121147)

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ELANE Antibody

Purpose: ELNE Antibody

Specificity: ELNE Polyclonal Antibody detects endogenous levels of protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: ELNE

Background:

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016

Gene Name: ELANE ELA2

Protein Name: Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase)

Gene ID: 1991

UniProt: P08246