FEN1 antibody (pSer187)

Catalog No. ABIN8121471

The Rabbit Polyclonal anti-FEN1 antibody is suitable to detect FEN1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for FEN1 antibody (pSer187) (ABIN8121471)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FEN1 Antibody

Binding Specificity: pSer187

Purpose: Rad2/FEN1 (Phospho Ser187) Antibody

Specificity: This antibody detects endogenous levels of Rad2/FEN1 (Phospho-Ser187) at Human, Mouse,Rat

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human Rad2/FEN1 (Phospho-Ser187)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: Rad2/FEN1

Background:

Synonyms: Flap endonuclease 1 (FEN-1) (EC 3.1.-.-) (DNase IV) (Flap structure-specific endonuclease 1) (Maturation factor 1) (MF1) (hFEN-1)

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage byThe protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions

Gene Name: FEN1 RAD2

Protein Name: Rad2/FEN1 (Phospho-Ser187)

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA