FOXO4 antibody (acLys407)

Catalog No. ABIN8121514

The Rabbit Polyclonal anti-FOXO4 antibody (ABIN8121514) specifically detects FOXO4 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for FOXO4 antibody (acLys407) (ABIN8121514)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FOXO4 Antibody

Binding Specificity: acLys407

Purpose: FoxO4 (Acetyl Lys407) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse FoxO4 (Acetyl Lys407)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human FoxO4 (Acetyl Lys407)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: FoxO4

Background:

Synonyms: Forkhead box protein O4 (Fork head domain transcription factor AFX1)

Background: disease:A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X,11)(q13,q23) with MLL/HRX. The result is a rogue activator protein.,function:Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.,pharmaceutical:A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity.,PTM:Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.,PTM:Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.,subunit:Interacts with CBP, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding.,tissue specificity:Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.,

Gene Name: FOXO4 AFX AFX1 MLLT7

Protein Name: FoxO4 (Acetyl Lys407)

Gene ID: 4303

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation