Connexin 43/GJA1 antibody (pSer265)

Catalog No. ABIN8121597

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody is suitable to detect Connexin 43/GJA1 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for Connexin 43/GJA1 antibody (pSer265) (ABIN8121597)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer265

Purpose: Connexin 43 (phospho Tyr265) Antibody

Specificity: Phospho-Connexin 43 (S265) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S265.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Connexin 43 (phospho Ser265)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Background:

Synonyms: GJA1, GJAL, Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein

Background: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014],

Gene Name: GJA1

Protein Name: Gap junction alpha-1 protein

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization