IDH3A antibody

Catalog No. ABIN8122372

The Rabbit Polyclonal anti-IDH3A antibody (ABIN8122372) specifically detects IDH3A in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IDH3A antibody (ABIN8122372)

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-IDH3A Antibody

Purpose: IDH3A Antibody

Specificity: IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human IDH3A.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for IDH3A

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Alternative Name: IDH3A

Background:

Synonyms: IDH3A, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008],

Gene Name: IDH3A

Protein Name: Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial

Gene ID: 3419

UniProt: P50213