LAT antibody (pTyr255)

Catalog No. ABIN8122660

The Rabbit Polyclonal anti-LAT antibody (ABIN8122660) specifically detects LAT in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LAT antibody (pTyr255) (ABIN8122660)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LAT Antibody

Binding Specificity: pTyr255

Purpose: LAT (Phospho Tyr255) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat LAT (Phospho Tyr255)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human LAT (Phospho Tyr255)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background:

Synonyms: Linker for activation of T-cells family member 1 (36 kDa phospho-tyrosine adapter protein, pp36, p36-38)

Background: function:Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.,miscellaneous:Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT.,PTM:Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.,PTM:Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation, which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement.,subcellular location:Present in lipid rafts.,subunit:When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A.,tissue specificity:Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).,

Gene Name: LAT

Protein Name: LAT (Phospho Tyr255)

Gene ID: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway