PKC antibody (AA 450-550)

Catalog No. ABIN8124064

The Mouse Monoclonal anti-PKC antibody (Clone AB2366) (ABIN8124064) specifically detects PKC in WB, IF and ELISA. The antibody is reactive with Human, Mouse, Rat, Cow and Rabbit samples.

Quick Overview for PKC antibody (AA 450-550) (ABIN8124064)

Target: PKC (Protein Kinase C (PKC))

Reactivity: Human, Mouse, Rat, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: AB2366

Product Details anti-PKC Antibody

Binding Specificity: AA 450-550

Purpose: PKC mouse mAb

Specificity: This antibody detects endogenous levels of PKC protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 450 - 550 of the human PKC protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for PKC

Target: PKC (Protein Kinase C (PKC))

Alternative Name: PKC

Background:

Synonyms: Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA, Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1, Protein kinase C gamma type, PKC-gamma, PRKCG, PKCG

Background: protein kinase C alpha (PRKCA) Homo sapiens Protein kinase C (PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca (2+) handling in myocytes. [provided by RefSeq, Jul 2

Gene Name: PRKCA PRKCB PRKCG

Protein Name: PKC

Gene ID: 5578

UniProt: P17252

Pathways: TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Phototransduction