RPA2 antibody

Catalog No. ABIN8124318

The Rabbit Monoclonal anti-RPA2 antibody is suitable to detect RPA2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for RPA2 antibody (ABIN8124318)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1892

Product Details anti-RPA2 Antibody

Purpose: RPA32/RPA2 Rabbit mAb

Specificity: This antibody detects endogenous levels of RPA32/RPA2 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA32/RPA2

Background:

Synonyms: RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34

Background: function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. , PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis) . Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. , subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. , subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. ,

Gene Name: RPA2

Protein Name: RPA32/RPA2

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA