RPS6 antibody (pSer240, pSer244)

Catalog No. ABIN8124409

The Rabbit Monoclonal anti-RPS6 antibody (Clone AB1610) (ABIN8124409) specifically detects RPS6 in WB, IHC, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RPS6 antibody (pSer240, pSer244) (ABIN8124409)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1610

Product Details anti-RPS6 Antibody

Binding Specificity: pSer240, pSer244

Purpose: RPS6 (Phospho Ser240/Ser244) Rabbit mAb

Specificity: The antibody detects endogenous levels of RPS6 protein only when phosphorylated at Ser240 and Ser244. The antibody does not cross-react with RPS6 phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6

Background:

Synonyms: RPS6, OK/SW-cl. 2, 40S ribosomal protein S6, Phosphoprotein NP33

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed

Gene Name: RPS6

Protein Name: RPS6 (Phospho Ser240/Ser244)

Gene ID: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly