MSK1 antibody (pSer360)

Catalog No. ABIN8124424

The Rabbit Monoclonal anti-MSK1 antibody (Clone AB1750) (ABIN8124424) specifically detects MSK1 in WB, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MSK1 antibody (pSer360) (ABIN8124424)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1750

Product Details anti-MSK1 Antibody

Binding Specificity: pSer360

Purpose: MSK1 (phospho Ser360) Rabbit mAb

Specificity: The antibody detects endogenous levels of MSK1 protein only when phosphorylated at Ser360. The antibody does not cross-react with MSK1 phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: MSK1

Background:

Synonyms: RPS6KA5, MSK1, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen-and stress-activated protein kinase 1, RSK-like protein kinase, RSKL

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein. , cofactor:Magnesium. , enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process. , function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1) . Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) , which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen-and stress-induced phosphorylation of high mobility group protein 14 (HMG-14) . , miscellaneous:Enzyme activity requires the presence of both kinase domains. , PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. , similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. , similarity:Contains 1 AGC-kinase C-terminal domain. , similarity:Contains 2 protein kinase domains. , subcellular location:Predominantly nuclear. Partially cytoplasmic. , subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. , tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. ,

Gene Name: RPS6KA5

Protein Name: MSK1 (phospho Ser360)

Gene ID: 9252

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades