TP53BP1 antibody

Catalog No. ABIN8125482

The Rabbit Monoclonal anti-TP53BP1 antibody is suitable to detect TP53BP1 in samples from Human. It has been validated for WB, ELISA, IF and IP.

Quick Overview for TP53BP1 antibody (ABIN8125482)

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1953

Product Details anti-TP53BP1 Antibody

Purpose: 53BP1 Rabbit mAb

Specificity: This antibody detects endogenous levels of 53BP1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: 53BP1

Background:

Synonyms: TP53BP1, Tumor suppressor p53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1

Background: function:May have a role in checkpoint signaling during mitosis (By similarity) . Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. , PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. , PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. , similarity:Contains 2 BRCT domains. , subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. , subunit:Interacts with IFI202A (By similarity) . Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro) . Does not bind unmethylated histone H3. ,

Gene Name: TP53BP1

Protein Name: 53BP1

Gene ID: 7158

UniProt: Q12888

Pathways: DNA Damage Repair