CD56 antibody (Internal Region)

Catalog No. ABIN768541

Product Details anti-CD56 Antibody

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: CD56 / NCAM1 (isoform 2)

Sequence: KASWTRPEKQ ETLD

Specificity: This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.

Cross-Reactivity: Cow, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150-170 kDa band observed in Human Cerebellum and Frontal Cortex lysates (calculated MW of 94.6 kDa according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form and is routinely observed by other source

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM1 (NCAM1 Products)

Synonyms: CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody

Background: NCAM1, neural cell adhesion molecule 1, CD56, MSK39, NCAM, N-CAM-1, NCAM-1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM

Molecular Weight: 94.6kDa according to NP_851996.2

Gene ID: 4684, 24586

NCBI Accession: NP_851996