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MTHFD1L antibody (AA 535-538)

Verified MTHFD1L Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN768655
  • Target See all MTHFD1L Antibodies
    MTHFD1L (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1-Like (MTHFD1L))
    Binding Specificity
    • 2
    • 1
    • 1
    • 1
    AA 535-538
    Reactivity
    Human, Mouse, Rat
    Host
    • 5
    • 3
    Goat
    Clonality
    • 6
    • 2
    Polyclonal
    Conjugate
    • 8
    This MTHFD1L antibody is un-conjugated
    Application
    • 7
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    MTHFD1L (aa535-538)
    Sequence
    DTNDRFLRKI TIGQ
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-DTNDRFLRKITIGQ, from the internal region of the protein sequence according to NP_056255.2.
    Isotype
    IgG
    Top Product
    Discover our top product MTHFD1L Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 110 kDa band observed in Human Brain (Cerebellum) (calculated MW of 106 kDa according to NP_056255.2). The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3 μg/mL. Prima

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    MTHFD1L (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1-Like (MTHFD1L))
    Alternative Name
    MTHFD1L (MTHFD1L Products)
    Synonyms
    FTHFSDC1 antibody, MTC1THFS antibody, dJ292B18.2 antibody, Fthfsdc1 antibody, 2410004L15Rik antibody, AI647056 antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like antibody, MTHFD1L antibody, Mthfd1l antibody
    Background
    MTHFD1L, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, dJ292B18.2, 10-formyl-THF synthetase, formyltetrahydrofolate synthetase domain containing 1, mitochondrial C1-tetrahydrofolate synthase
    Molecular Weight
    106kDa according to NP_056255.2
    Gene ID
    25902, 270685, 361472
    NCBI Accession
    NP_056255
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