Insulin antibody

Catalog No. ABIN7477942

The Mouse Monoclonal anti-Insulin antibody (Clone 6E9F1) (ABIN7477942) specifically detects Insulin in ELISA. The antibody is reactive with Human samples.

Quick Overview for Insulin antibody (ABIN7477942)

Target: Insulin (INS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: ELISA

Clone: 6E9F1

Product Details anti-Insulin Antibody

Specificity: Insulin Antibody (6E9F1), mAb, Mouse detects human Insulin and slightly recognizes proinsulin, it has no cross-reactivity with C-peptide.

Cross-Reactivity (Details): Insulin Antibody (6E9F1), mAb, Mouse detects human Insulin and slightly recognizes proinsulin, it has no cross-reactivity with C-peptide.

Purification: Protein A affinity column

Immunogen: human recombinant Insulin expressed in yeast

Isotype: IgG2a

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/mL ELISA Detection: 0.05-0.2 µg/mL Recommended antibody pairing for sandwich immunoassay: Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Proinsulin is the prohormone precursor to insulin made in pancreas. It is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids, 4 are removed altogether, and the remaining 31 form the C-Peptide. The C-Peptide is abstracted from the center of the proinsulin sequence, the two other ends (alpha and beta chains) remain connected by disulfide bonds. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma. Insulin Antibody (6E9F1), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant Insulin expressed in yeast.

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation