PLA2G4F antibody (AA 781-795)
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- Target See all PLA2G4F products
- PLA2G4F (Phospholipase A2, Group IVF)
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Binding Specificity
- AA 781-795
- Reactivity
- Human, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PLA2G4F antibody is un-conjugated
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Application
- ELISA
- Specificity
- Human PLA2G4F / CPLA2-zeta.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%) Gorilla, Elephant (93%) Mouse, Bat (87%) Hamster, Bovine (80%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-QTAEEKAFGDFVINR from an internal region (near C-terminus) of human PLA2G4F / CPLA2-zeta (NP_998765.3). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla, Elephant (93%), Mouse, Bat (87%), Hamster, Bovine (80%).
Type of Immunogen: Synthetic peptide
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- Application Notes
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Approved: Peptide-ELISA (1:16000)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 55-60kD and 35kD in Human Placenta lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kD according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PLA2G4F (Phospholipase A2, Group IVF)
- Alternative Name
- PLA2G4F (PLA2G4F Products)
- Synonyms
- PLA2G4FZ antibody, 4732472I07Rik antibody, Gm647 antibody, Pla2zeta antibody, phospholipase A2 group IVF antibody, phospholipase A2, group IVF antibody, PLA2G4F antibody, Pla2g4f antibody
- Background
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Name/Gene ID: PLA2G4F
Synonyms: PLA2G4F, CPLA2-zeta, Phospholipase A2 group IVF, Phospholipase A2, group IVF, PLA2G4FZ, PLA2G4F/Z - Gene ID
- 255189
- NCBI Accession
- NP_998765
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