Ataxin 1 antibody (AA 686-699)

Catalog No. ABIN793324

Product Details anti-Ataxin 1 Antibody

Target: Ataxin 1 (ATXN1)

Binding Specificity: AA 686-699

Reactivity: Human, Mouse, Rat, Monkey, Cow, Pig, Horse, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Ataxin 1 antibody is un-conjugated

Application: ELISA

Specificity: Human ATXN1 / Ataxin-1. Reported variants represent identical protein: NP_001121636.1, NP_000323.2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Pig, Opossum (100%) Turkey, Chicken, Lizard, Xenopus (93%) Panda, Dog, Platypus, Pufferfish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TLKNLKNGSVKKGQ from an internal region of human ATXN1 / Ataxin-1 (NP_000323.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Pig, Opossum (100%), Turkey, Chicken, Lizard, Xenopus (93%), Panda, Dog, Platypus, Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75kD and 26kD in Mouse Fetal Brain lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kD according to NP_001186234.1 and 83.8kD according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Ataxin 1

Target: Ataxin 1 (ATXN1)

Alternative Name: ATXN1 / Ataxin-1 / SCA1 (ATXN1 Products)

Synonyms: ATX1 antibody, D6S504E antibody, SCA1 antibody, ATXN1 antibody, ataxin 1b antibody, atxn1 antibody, 2900016G23Rik antibody, Atx1 antibody, C85907 antibody, ENSMUSG00000074917 antibody, Gm10786 antibody, Sca1 antibody, CG4547 antibody, Dmel\\CG4547 antibody, dAtx-1 antibody, dAtx1 antibody, sca1 antibody, ataxin 1 antibody, ataxin 1b antibody, Ataxin 1 antibody, ATXN1 antibody, atxn1b antibody, Atxn1 antibody, Atx-1 antibody

Background: Name/Gene ID: ATXN1

Synonyms: ATXN1, ATX1, Ataxin 1, D6S504E, Ataxin-1, SCA1

Gene ID: 6310

NCBI Accession: NP_000323

UniProt: P54253

Pathways: Synaptic Membrane