ERK1 antibody (pTyr204) (AbBy Fluor® 647)

Catalog No. ABIN879227

The Rabbit Polyclonal anti-ERK1 antibody has been validated for IF (p) and IF (cc). It is suitable to detect ERK1 in samples from Mouse.

Quick Overview for ERK1 antibody (pTyr204) (AbBy Fluor® 647) (ABIN879227)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is conjugated to AbBy Fluor® 647

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-ERK1 Antibody (AbBy Fluor® 647)

Binding Specificity: pTyr204

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MAPK3 around the phosphorylation site of Tyr204

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background:

Synonyms: MAPK3phospho Y204, ERK/MAPKphospho T202/Y204, p44/42 MAP KinaseThr202, ERK 1, ERK, ERK-1, ERK1, Extracellular Signal Regulated Kinase 1, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, MAP kinase 1, MK03_RAT, MAP Kinase, MAP kinase isoform p44, MAPK 1, MAPK, MAPK1, MAPK3, MGC20180, Mitogen-activated protein kinase 1, OTTHUMP00000174538, OTTHUMP00000174541, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK,

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Gene ID: 5595

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins