Bcl-2 antibody (AA 161-239) (AbBy Fluor® 488)

Catalog No. ABIN884674

This Rabbit Polyclonal antibody specifically detects Bcl-2 in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Rabbit and Sheep.

Quick Overview for Bcl-2 antibody (AA 161-239) (AbBy Fluor® 488) (ABIN884674)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Bcl-2 Antibody (AbBy Fluor® 488)

Binding Specificity: AA 161-239

Cross-Reactivity: Human, Mouse, Pig, Rabbit, Rat, Sheep

Predicted Reactivity: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background:

Synonyms: Bcl-2, PPP1R5, Apoptosis regulator Bcl-2, BCL2

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling