C-Ski/V-Ski (AA 551-650) antibody (Alexa Fluor 488)
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- Target
- C-Ski/V-Ski
- Binding Specificity
- AA 551-650
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Alexa Fluor 488
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Predicted Reactivity
- Human,Mouse,Rat,Dog,Cow,Horse,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human C-SKI
- Isotype
- IgG
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- C-Ski/V-Ski
- Background
-
Synonyms: Sloan-kettering viral oncogene homolog, Avian sarcoma viral v ski oncogene homolog, C oncogene, C ski, Ski oncogene, Ski oncoprotein, SKV, v ski avian sarcoma viral oncogene homolog, v ski sarcoma viral oncogene homolog, v-ski sarcoma viral oncogene homolog, SKI_HUMAN.
Background: SKI is a nuclear protein that forms homodimers and heterodimers and binds to DNA to function as transcriptional activators and repressors. The SKI oncoprotein dramatically affects cell growth, differentiation, and/or survival. SKI was shown to act in distinct signaling pathways including those involving nuclear receptors, transforming growth factor beta, and tumor suppressors. SKI inhibits transforming growth factor-beta (TGF-beta) signaling through interaction with Smad proteins. SKI represses Smad-mediated transcriptional activation, probably through its action as a transcriptional co-repressor. SKI also inhibits TGF-beta-induced downregulation of genes such as c-myc.
- Gene ID
- 6497
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