DNASE1 antibody (AA 101-200) (HRP)

Catalog No. ABIN894181

Product Details anti-DNASE1 Antibody (HRP)

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Binding Specificity: AA 101-200

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNase I

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: DNase 1 (DNASE1 Products)

Synonyms: LOC397802 antibody, DNL1 antibody, DRNI antibody, AI788650 antibody, DNaseI antibody, Dnl1 antibody, dnase1-A antibody, zgc:92440 antibody, DNase I antibody, deoxyribonuclease 1 antibody, deoxyribonuclease I antibody, deoxyribonuclease I L homeolog antibody, Deoxyribonuclease I antibody, endA antibody, LOC397802 antibody, DNASE1 antibody, Dnase1 antibody, dnase1.L antibody, dnase1 antibody, CpipJ_CPIJ011184 antibody, CpipJ_CPIJ011185 antibody, CpipJ_CPIJ011186 antibody, CpipJ_CPIJ011187 antibody, CpipJ_CPIJ011190 antibody, CpipJ_CPIJ017803 antibody, Desal_1409 antibody, MPET_RS05255 antibody, METEV_RS02275 antibody, Plabr_0631 antibody

Background:

Synonyms: RNASE1, Dornase alfa, Deoxyribonuclease 1, Deoxyribonuclease I, Deoxyribonuclease1, DeoxyribonucleaseI, DNASE 1, DNase I lysosomal, DNASE1, DNaseI, DNL 1, DNL1, DRNI, Human urine deoxyribonuclease I, DNAS1_HUMAN.

Background: Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.

Gene ID: 1773