KLHL13 antibody (AA 351-450) (HRP)

Catalog No. ABIN903880

Product Details anti-KLHL13 Antibody (HRP)

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Binding Specificity: AA 351-450

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLHL13 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Zebrafish (Danio rerio)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KLHL13

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KLHL13

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Alternative Name: KLHL13 (KLHL13 Products)

Synonyms: 1200009K10Rik antibody, AI451128 antibody, Bklhd2 antibody, D130072F20Rik antibody, mKIAA1309 antibody, BKLHD2 antibody, Kelch-like protein 13 antibody, kelch-like 13 antibody, kelch like family member 13 antibody, kelch like family member 9 antibody, kelch-like family member 13 antibody, klh13 antibody, Klhl13 antibody, KLHL13 antibody, KLHL9 antibody

Background:

Synonyms: Kelch-like protein 13, BTB and kelch domain-containing protein 2, KLH13_HUMAN.

Background: KLHL13 (kelch like 13), also known as BKLHD2 (BTB and kelch domain containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate specific adapter of an E3 ubiquitin protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin mediated proteolysis. Via its BTB and C terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL3 ligase.

Gene ID: 90293