PDPK1 antibody (AA 251-350) (AbBy Fluor® 350)

Catalog No. ABIN910584

The AbBy Fluor® 350-conjugated Rabbit Polyclonal anti-PDPK1 antibody (ABIN910584) specifically detects PDPK1 in WB, IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for PDPK1 antibody (AA 251-350) (AbBy Fluor® 350) (ABIN910584)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PDPK1 Antibody (AbBy Fluor® 350)

Binding Specificity: AA 251-350

Purpose: PDPK1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PDK1

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1

Background:

Synonyms: 3 Phosphoinositide Dependent Protein Kinase 1, hPDK 1, PDK 1, PDPK1, PkB kinase, PkB kinase like gene 1, PkB like 1, PDPK1_HUMAN.

Background: PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.

Gene ID: 5170

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals