TNF alpha antibody (Biotin)

Catalog No. ABIN918027

The Biotin-conjugated Rabbit Polyclonal anti-TNF alpha antibody (ABIN918027) specifically detects TNF alpha in WB and IHC (p). The antibody is reactive with Human, Pig and Dog samples.

Quick Overview for TNF alpha antibody (Biotin) (ABIN918027)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TNF alpha Antibody (Biotin)

Cross-Reactivity: Dog, Human, Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from dog TNF-alpha

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background:

Synonyms: APC1, Cachectin, D, Dferentiation inducing factor, Macrophage cytotoxic factor, MCF, Necrosin, TNF a, TNF alpha, TNF, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor, Tumour Necrosis Factor Alpha, TNFA_HUMAN.

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Molecular Weight: 17/26kDa

Gene ID: 403922

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome