Uhrf1/2 (AA 701-793) antibody (Cy7)

Catalog No. ABIN919289

Product Details

Target: Uhrf1/2

Binding Specificity: AA 701-793

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Cy7

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UHRF1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Uhrf1/2

Background:

Synonyms: HuNp95, ICBP90, Inverted CCAAT box binding protein of 90 kDa, Inverted CCAAT box binding protein, 90-kD, Inverted CCAAT box-binding protein of 90 kDa, Liver regeneration-related protein LRRG126, NP95, Nuclear phosphoprotein, 95-KD, Nuclear protein 95, Nuclear zinc finger protein Np95, RING finger protein 106, RNF106, Transcription factor ICBP90, Ubiquitin like containing PHD and RING finger domains protein 1, Ubiquitin like PHD and RING finger domain containing protein 1, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like protein containing PHD and RING finger domains 1, Ubiquitin-like with PHD and ring finger domains 1, Ubiquitin-like, containing PHD and RING finger domains, 1, Ubiquitin-like-containing PHD and RING finger domains protein 1, UHRF1, UHRF1_HUMAN, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like-containing PHD and RING finger domains protein 2, UHRF2, UHRF2_HUMAN, E3 ubiquitin-protein ligase UHRF2.

Background: Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.Tissue specificity:Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. UHRF2 is an E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131.

Gene ID: 29128, 115426