AIF antibody (N-Term)

Catalog No. ABIN926855

The Rabbit Polyclonal anti-AIF antibody has been validated for WB and IHC. It is suitable to detect AIF in samples from Human, Cow and Dog.

Quick Overview for AIF antibody (N-Term) (ABIN926855)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AIF Antibody

Binding Specificity: N-Term

Cross-Reactivity: Cow (Bovine), Dog (Canine)

Purification: Purified

Immunogen: PDCD8 antibody was raised in rabbit using the N terminal of PDCD8 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PDCD8 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this PDCD8 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: PDCD8

Background: AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Synonyms: Polyclonal PDCD8 antibody, Anti-PDCD8 antibodyRP3-438D16.2 antibody, AIF antibody, MGC111425 antibody.

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect