AKT2 antibody (Middle Region)

Catalog No. ABIN927290

The Rabbit Polyclonal anti-AKT2 antibody is suitable to detect AKT2 in samples from Human, Mouse, Rat, Dog, Chicken, Cow, Pig, Xenopus laevis and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for AKT2 antibody (Middle Region) (ABIN927290)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Pig, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKT2 Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Frog, Chicken, Dog (Canine), Cow (Bovine), Zebrafish (Brachydanio rerio), Pig (Porcine)

Purification: Purified

Immunogen: AKT2 antibody was raised in rabbit using the middle region of AKT2 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

AKT2 Blocking Peptide, (ABIN5611998), is also available for use as a blocking control in assays to test for specificity of this AKT2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2

Background: AKT2 belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene encoding AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins. Synonyms: Polyclonal AKT2 antibody, Anti-AKT2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody.

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling