Estrogen Receptor alpha antibody (C-Term)

Catalog No. ABIN927316

This Rabbit Polyclonal antibody specifically detects Estrogen Receptor alpha in WB. It exhibits reactivity toward Human.

Quick Overview for Estrogen Receptor alpha antibody (C-Term) (ABIN927316)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: C-Term

Purification: Purified

Immunogen: ESR1 antibody was raised in rabbit using the C terminal of ESR1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

ESR1 Blocking Peptide, (ABIN940517), is also available for use as a blocking control in assays to test for specificity of this ESR1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background: ESR1 is an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. Synonyms: Polyclonal ESR1 antibody, Anti-ESR1 antibody, estrogen receptor 1 antibody, DKFZp686N23123 antibody, ER antibody, ESR antibody, ESRA antibody, Era antibody, NR3A1 antibody.

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly