WT1 antibody (N-Term)

Catalog No. ABIN927690

Product Details anti-WT1 Antibody

Target: WT1 (Wilms Tumor 1 (WT1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: WT1 antibody was raised in rabbit using the N terminal of WT1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

WT1 Blocking Peptide, catalog no. 33R-6321, is also available for use as a blocking control in assays to test for specificity of this WT1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 (WT1 Products)

Synonyms: AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

Background: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Synonyms: Polyclonal WT1 antibody, Anti-WT1 antibody, Wilms tumor 1 antibody, AWT1 antibody, GUD antibody, WAGR antibody, WIT-2 antibody, WT33 antibody.

Pathways: Tube Formation