SHC1 antibody (C-Term)

Catalog No. ABIN928366

This Rabbit Polyclonal antibody specifically detects SHC1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Chicken and Xenopus laevis.

Quick Overview for SHC1 antibody (C-Term) (ABIN928366)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHC1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Frog, Chicken, Dog (Canine), Zebrafish (Brachydanio rerio)

Purification: Purified

Immunogen: SHC1 antibody was raised in rabbit using the C terminal of SHC1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

SHC1 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this SHC1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: SHC1

Background: SHC1 is a signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. Synonyms: Polyclonal SHC1 antibody, Anti-SHC1 antibody, SHC, Src homology 2 domain containing transforming protein 1 antibody, FLJ26504 antibody, SHC antibody, SHCA antibody, p52SHC antibody, p66 antibody, p66SHC antibody.

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2