CBFA2T2 antibody

Catalog No. ABIN930909

The Mouse Monoclonal anti-CBFA2T2 antibody (Clone CBF5I134) (ABIN930909) specifically detects CBFA2T2 in WB, ICC, DB and FACS. The antibody is reactive with Human samples.

Quick Overview for CBFA2T2 antibody (ABIN930909)

Target: CBFA2T2 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBFA2T2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Flow Cytometry (FACS)

Clone: CBF5I134

Product Details anti-CBFA2T2 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: CBFA2 T2 antibody was raised in mouse using recombinant Core-Binding Factor,Alpha Subunit 2

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: CBFA2 T2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for CBFA2T2

Target: CBFA2T2 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2))

Alternative Name: CBFA2T2

Background: In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. Synonyms: Monoclonal CBFA2T2 antibody, Anti-CBFA2T2 antibody, Core-binding factor runt domain alpha subunit 2 antibody, MTGR1 antibody, EHT antibody, ZMYND3 antibody.