Vimentin antibody

Catalog No. ABIN931335

The Mouse Monoclonal anti-Vimentin antibody (Clone 9E7E7-5G3F10-5G3F10E5) (ABIN931335) specifically detects Vimentin in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Vimentin antibody (ABIN931335)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 9E7E7-5G3F10-5G3F10E5

Product Details anti-Vimentin Antibody

Immunogen: Vimentin antibody was raised in Mouse using a purified recombinant fragment of Vimentin expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin is a member of the intermediate filament family of proteins that is especially found in connective tissue. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. This filament is used as a marker for mesodermally derived tissues, and as such can be used as an immunohistochemical marker for sarcomas. Synonyms: Monoclonal Vimentin antibody, Anti-Vimentin antibody.

Pathways: Caspase Cascade in Apoptosis