SIV p28 antibody (HRP)
SIV p28
Reactivity: Simian Immunodeficiency Virus (SIV)
ELISA, WB
Host: Mouse
Monoclonal
3F7
HRP
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- Target
- SIV p28 (Simian Immunodeficiency Virus p28 (SIV p28))
- Reactivity
- Simian Immunodeficiency Virus (SIV)
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- This SIV p28 antibody is conjugated to HRP
- Application
- ELISA, Western Blotting (WB)
- Purification
- purified
- Immunogen
- SIV mac251 p28 antibody was raised in mouse using SIV mac251 p28 as the immunogen.
- Clone
- 3F7
- Isotype
- IgG1
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- Application Notes
-
ELISA: 1:1000, WB: 1:500-1:1000
Optimal conditions should be determined by the investigator. - Comment
-
Binds to recombinant p28 (SIV mac251), not epitope mapped.
- Restrictions
- For Research Use only
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- Concentration
- Lot specific
- Buffer
- Supplied as a purified IgG in 50 mM Tris buffer, pH 8.0, with no preservatives added. The HRP is attached by glutaraldehyde coupling to the lysine residue.
- Preservative
- Without preservative
- Precaution of Use
- Not tested for sterility or endotoxin content.
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use. - Storage
- 4 °C/-80 °C
- Storage Comment
- Store lyophilized product at 2-8 °C short term, following reconstitution, aliquot and store at -70 °C or lower.
-
- Target
- SIV p28 (Simian Immunodeficiency Virus p28 (SIV p28))
- Target Type
- Viral Protein
- Background
- HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells and dendritic cells, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4. This inactivates the host adaptive immune response. Synonyms: Monoclonal SIV mac251 gp28 antibody, Anti-SIV mac251 gp28 antibody, gp28 antibody, Envelope surface glycoprotein gp28 antibody, Glycoprotein 28 antibody, gp28 antibody, gp28 glycoprotein antibody, Simian Immunodeficiency Virus 1 antibody, SU antibody, Surface protein antibody.
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