beta-2 Microglobulin antibody (beta-2-Microglobulin) (Biotin)

Details for Product anti-B2M Antibody No. ABIN93901
Antigen
Reactivity
Human, Pig (Porcine)
486
100
67
64
16
8
6
4
3
2
2
2
1
1
1
1
1
1
Host
Mouse
322
195
15
11
9
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta-2 Microglobulin antibody is conjugated to Biotin
49
34
18
18
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
264
249
183
159
126
95
60
43
40
33
32
24
19
13
8
8
8
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Options
Immunogen Purified isolated human beta2-microglobulin.
Clone B2M-02
Isotype IgG1
Specificity The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Cross-Reactivity (Details) Human, Porcine
Antigen
Alternative Name beta2-Microglobulin (B2M Antibody Abstract)
Background Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-beta-2-Microglobulin (B2M) antibody (Biotin) (ABIN93901) Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-micro...
Product cited in: Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, 2001 (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, 1985 (PubMed).