Endoglin (ENG) antibody (FITC)

Details for Product No. ABIN93940
Antigen
Reactivity
Human, Rat (Rattus)
455
174
82
19
14
8
5
5
5
4
1
Host
Mouse
260
249
47
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
56
51
46
22
8
8
7
7
7
7
7
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
352
340
130
115
79
72
47
38
26
21
17
13
12
7
7
6
4
3
3
2
2
2
2
1
Options
Immunogen Recombinant Vaccinia virus containing the human CD105 cDNA.
Clone MEM-226
Isotype IgG2a
Specificity The antibody MEM-226 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Cross-Reactivity (Details) Human, Rat
Antigen
Alternative Name CD105 (ENG Antibody Abstract)
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Endoglin (ENG) antibody (FITC) (ABIN93940) Surface staining (flow cytometry) of HUVEC (human umbilical vein endothelial cells) w...
Image no. 2 for anti-Endoglin (ENG) antibody (FITC) (ABIN93940) Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 () 143Nd. ...
Product cited in: Barale, Dentelli, Togliatto, Trombetta, Olgasi, Scozzari, Toppino, Morino, Brizzi: "High glucose via NOX-dependent ROS generation and AKT activity promotes adipose-derived stem cell de-differentiation." in: Stem cells and development, 2012 (PubMed).

Schmidt, Achermann, Odermatt, Breymann, Mol, Genoni, Zund, Hoerstrup: "Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source." in: Circulation, Vol. 116, Issue 11 Suppl, pp. I64-70, 2007 (PubMed).