CD46 antibody

Catalog No. ABIN94149

This anti-CD46 antibody is a Mouse Monoclonal antibody detecting CD46 in WB, FACS and IP. Suitable for Human and Cow. This Primary Antibody has been cited in 8+ publications.

Quick Overview for CD46 antibody (ABIN94149)

Target: CD46

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-258

Product Details anti-CD46 Antibody

Purpose: Anti-Hu CD46 Purified

Specificity: The antibody MEM-258 recognizes an extracellular epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast, it is negative on erythrocytes.

Cross-Reactivity (Details): Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL human T cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD46 antibody (ABIN94149)

Kälin, Amstutz, Gastaldelli, Wolfrum, Boucke, Havenga, DiGennaro, Liska, Hemmi, Greber: "Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35." in: Journal of virology, Vol. 84, Issue 10, pp. 5336-50, (2010) (PubMed).

Wang, Tuve, Erdman, Lieber: "Receptor usage of a newly emergent adenovirus type 14." in: Virology, Vol. 387, Issue 2, pp. 436-41, (2009) (PubMed).

Hoffmann, Bayer, Heim, Potthoff, Nettelbeck, Wildner: "Evaluation of twenty-one human adenovirus types and one infectivity-enhanced adenovirus for the treatment of malignant melanoma." in: The Journal of investigative dermatology, Vol. 128, Issue 4, pp. 988-98, (2008) (PubMed).

Fleischli, Sirena, Lesage, Havenga, Cattaneo, Greber, Hemmi: "Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor." in: The Journal of general virology, Vol. 88, Issue Pt 11, pp. 2925-34, (2007) (PubMed).

Loré, Adams, Havenga, Precopio, Holterman, Goudsmit, Koup: "Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1721-9, (2007) (PubMed).

Fremeaux-Bacchi, Moulton, Kavanagh, Dragon-Durey, Blouin, Caudy, Arzouk, Cleper, Francois, Guest, Pourrat, Seligman, Fridman, Loirat, Atkinson: "Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 2017-25, (2006) (PubMed).

Weyand, Lee, Higashi, Cawley, Yoshihara, So: "Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies." in: Infection and immunity, Vol. 74, Issue 4, pp. 2428-35, (2006) (PubMed).

Fleischli, Verhaagh, Havenga, Sirena, Schaffner, Cattaneo, Greber, Hemmi: "The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35." in: Journal of virology, Vol. 79, Issue 15, pp. 10013-22, (2005) (PubMed).

Target Details for CD46

Target: CD46

Alternative Name: CD46

Background: CD46 Molecule,CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.,MCP, TLX, AHUS2, MIC10, MGC26544

Gene ID: 4179

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization