ICAM-1 antibody (Intercellular Adhesion Molecule 1) (Biotin)

Details for Product anti-ICAM1 Antibody No. ABIN94179
Antigen
Reactivity
Human
611
113
83
15
14
8
4
3
1
1
1
1
Host
Mouse
398
282
55
7
5
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ICAM-1 antibody is conjugated to Biotin
71
65
55
24
11
10
9
7
6
6
6
6
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
390
358
210
187
140
87
69
61
50
37
28
22
21
17
11
9
6
4
3
2
2
1
1
1
1
1
1
Options
Immunogen Raji cells and spleen cells fused with NS1 cells
Clone 1H4
Isotype IgG2b
Specificity The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression of CD54 is upregulated by activation.
Cross-Reactivity (Details) Other not tested, Human
Antigen
Alternative Name CD54 (ICAM1 Antibody Abstract)
Background CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Intercellular Adhesion Molecule 1 (ICAM1) antibody (Biotin) (ABIN94179) Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1...
Product cited in: Williams, Chaudhry, Goodfellow, Lea, Evans: "Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites." in: The Journal of general virology, Vol. 85, Issue Pt 3, pp. 731-8, 2004 (PubMed).