Transferrin Receptor (p90, CD71) (TFRC) antibody (PE)

Details for Product No. ABIN94224
Antigen
Reactivity
Human
575
166
47
11
6
6
5
5
5
4
3
2
1
1
Host
Mouse
450
156
108
7
Clonality (Clone)
Monoclonal ()
Conjugate
PE
73
63
61
30
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
482
281
187
176
141
109
57
45
42
25
12
8
8
6
2
2
2
1
1
1
1
Options
Immunogen NALM-6 human pre-B cell line
Clone MEM-75
Isotype IgG1
Specificity The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes.
The antibody MEM-75 does not block binding of transferrin to the receptor.
HLDA IV, WS Code A 45
HLDA V, WS Code T T-165
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD71 (TFRC Antibody Abstract)
Background CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.
Pathways Transition Metal Ion Homeostasis
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (PE) (ABIN94224) Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with ...
Image no. 2 for anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (PE) (ABIN94224) Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green)...
Product cited in: Beck, Balogh, Kis, Izsépi, Cervenak, László, Bíró, Liliom, Mocsár, Vámosi, Füst, Matko: "New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production." in: Journal of lipid research, Vol. 51, Issue 2, pp. 286-96, 2010 (PubMed).