PAG1 antibody (AA 97-432)

Catalog No. ABIN94272

This anti-PAG1 antibody is a Mouse Monoclonal antibody detecting PAG1 in WB, IHC (p) and ICFC. Suitable for Human. This Primary Antibody has been cited in 5+ publications.

Quick Overview for PAG1 antibody (AA 97-432) (ABIN94272)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Flow Cytometry (ICFC)

Clone: MEM-255

Product Details anti-PAG1 Antibody

Binding Specificity: AA 97-432

Purpose: Anti-Hu PAG1 Purified

Specificity: The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

No Cross-Reactivity: Cow, Mouse, Rat

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil, spleen.
Western blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-PAG1 antibody (ABIN94272)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Yerly, Ding, Tauzin, van Echten-Deckert, Borisch, Hoessli: "The sphingolipid-rich rafts of ALK+ lymphomas downregulate the Lyn-Cbp/PAG signalosome." in: European journal of haematology, (2010) (PubMed).

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).

Brdicková, Brdicka, Andera, Spicka, Angelisová, Milgram, Horejsí: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, (2001) (PubMed).

Target Details for PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Alternative Name: PAG1

Background: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

Gene ID: 55824

UniProt: Q9NWQ8

Pathways: p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events